Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Ferula elaeochytris root extract on smooth muscle contraction of vas deferens gland in rat

Ozge Ozturk Cimentepe¹ , Cemil Gocmen¹, Nadire Eser², Hacer Sinem Buyuknacar³, Mehmet Cimentepe⁴

¹Department of Pharmacology, Faculty of Medicine, Cukurova University, Adana, Turkey; ²Department of Pharmacology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey; ³Department of Pharmacology, Faculty of Pharmacy, Cukurova University, Turkey; ⁴Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Harran University, Sanliurfa, Turkey.

For correspondence:- Ozge Cimentepe Email: ozge-ozturk01@hotmail.com Tel:+905369324011

Accepted: 23 June 2022 Published: 31 July 2022

Citation: Cimentepe OO, Gocmen C, Eser N, Buyuknacar HS, Cimentepe M. Effect of Ferula elaeochytris root extract on smooth muscle contraction of vas deferens gland in rat. Trop J Pharm Res 2022; 21(7):1499-1505 doi: 10.4314/tjpr.v21i7.21

© 2022 The authors.
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Abstract

Purpose: To evaluate the effect of Ferula elaeochytris (FE) at the prostatic and epididymal ends of rat vas deferens.

Methods: The effects of cumulative concentrations of FE (10 µL; 31.25 mg/µL and 20 µL; 62.5 mg/µL) were investigated on prostatic and epididymal ends of rat vas deferens in the presence of prazosin (0.3 μM), suramin (100 μM), atropine (10 nM) and nitric oxide synthase inhibitor (L-NOARG; 100 µM). The muscle contractions were induced by electrical field stimulation (EFS; 4 Hz, 50 V, 0.15 ms). Calcium (3 and 6 mM) was added into the bath medium while electrical field stimulation (EFS) was in progress.

Results: Ferula elaeochytris significantly inhibited the muscle contractions induced by electrical field stimulation (EFS) in a concentration-dependent manner. In the presence of prazosin or suramin, the contractile responses to EFS were significantly inhibited by FE at the prostatic and epididymal ends of vas deferens (p < 0.05). However, this inhibition was not affected by atropine and L- NOARG, suggesting that there is no direct interaction of FE with cholinergic and nitrergic responses. However, in the presence of prazosin or suramin, Ca²⁺ addition to the organ bath significantly reversed the inhibitory effect of FE at the prostatic and epididymal ends of vas deferens (p < 0.05).

Conclusion: These results show an inhibitory effect for the extract of FE on neurogenic contractile activity of prostatic and epididymal ends of vas deferens. This effect of FE may be associated with Ca²⁺ channels.

Keywords: Contractile activity, Electrical Field Stimulation (EFS), Ferula elaeochytris, Rat, Vas deferens